College of Osteopathic Medicine Faculty Articles

PubMed Identifier

2906327

Title

Multilocus molecular mapping of the mouse X chromosome.

ISBN or ISSN

0888-7543

Publication Title

Genomics

Volume

3

Issue

3

Publication Date / Copyright Date

10-1988

First Page

187

Last Page

194

Publisher

Academic Press

Abstract

Using restriction fragment length polymorphisms (RFLPs) and enzymatic variants between distantly related mouse species, we have assigned three genes to the mouse X chromosome and concurrently mapped a total of eight genes spanning an estimated 50 cM of the chromosome. Segregation of RFLPs in over 200 male progeny from interspecies backcrosses between the inbred strain C57BL/6JRos and either wild-derived Mus musculus or Mus spretus was followed for the murine genes Timp (tissue inhibitor of metalloproteinases), Cf-8 (coagulation factor VIII), and Rsvp (red-sensitive visual pigment) and the known X-linked markers Otc, Hprt, Cf-9, G6pd, and Ags. From the centromere, the gene order was defined as Otc, Timp, Hprt, Cf-9, (Cf-8/Rsvp/G6pd), Ags, by minimizing the number of multiple recombinational events. No significant differences in map order or frequency of recombination were observed between the two backcross series studied. The use of Southern analysis has allowed us to add new genes to the map in a cumulative manner, and as probes become available, additional markers can be mapped, using the same set of mice, by utilizing existing blots or resampling the DNAs. The use of probes for functional genes has allowed us to directly compare the X chromosomes of mouse and man and has provided insight into chromosomal rearrangements which have occurred during the evolutionary divergence of these species, as well as to define the extent of linkage homologies.

Disciplines

Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy

Keywords

Animals, Chromosome Mapping, Crosses, Genetic, Enzymes, Genes, Genetic Linkage, Genetic Variation, Mice, Mice, Inbred C57BL, Muridae, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, X Chromosome

This document is currently not available here.

Peer Reviewed

Find in your library

Share

COinS