College of Osteopathic Medicine Faculty Articles

PubMed Identifier

2341153

Title

Localization of the rhodopsin gene to the distal half of mouse chromosome 6.

ISBN or ISSN

0888-7543

Publication Title

Genomics

Volume

6

Issue

4

Publication Date / Copyright Date

4-1990

First Page

635

Last Page

644

Publisher

Academic Press

Abstract

We have assigned the mouse rhodopsin gene, Rho, to chromosome 6 using DNA from a set of mouse-hamster somatic hybrid cell lines and a partial cDNA clone for mouse opsin. This assignment rules out the direct involvement of the rhodopsin gene in the known mouse mutations that produce retinal degeneration, including retinal degeneration slow (rds, chromosome 17), retinal degeneration (rd, chromosome 5), Purkinje cell degeneration (pcd, chromosome 13), and nervous (nr, chromosome 8). Segregation of Rho-specific DNA fragment differences among 50 animals from an interspecific backcross (C57BL/6J X Mus spretus) X C57BL/6J indicates that the Rho locus is 4.0 +/- 2.8 map units distal to the locus for the proto-oncogene Raf-1 and 18.0 +/- 5.4 map units proximal to the locus for the proto-oncogene Kras-2. Linkage to Raf-1 was confirmed using four sets of recombinant inbred strains. The two loci RAF1 and RHO are also syntenic on human chromosome 3, but on opposite arms.

Disciplines

Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy

Keywords

Animals, Chromosome Inversion, Chromosome Mapping, Cricetinae, Cricetulus, Crosses, Genetic, Genetic Linkage, Humans, Hybrid Cells, Mice, Mice, Inbred Strains, Muridae, Phylogeny, Proto-Oncogenes, Recombination, Genetic, Restriction Mapping, Retinal Pigments, Rhodopsin, Species Specificity

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