College of Osteopathic Medicine Faculty Articles

PubMed Identifier

2110546

Title

Efficient linkage of 10 loci in the proximal region of the mouse X chromosome.

ISBN or ISSN

0888-7543

Publication Title

Genomics

Volume

7

Issue

1

Publication Date / Copyright Date

5-1990

First Page

19

Last Page

30

Publisher

Academic Press

Abstract

Interspecific Mus species crosses were used to construct a multilocus genetic map of the mouse X chromosome that extends for more than 50 cM. In these studies, we established the segregation of eight loci in more than 200 backcross progeny from crosses of M. musculus and M. spretus with a common inbred strain (C57BL/6JRos). Genetic divergence at the level of the nucleotide sequences makes these crosses a useful cumulative genetic resource for mapping additional genes defined by genomic or cDNA probes in a highly efficient manner. We have therefore devised a mapping strategy that uses a subset of these backcrosses that are recombinant between successive anchor loci to both localize and order an additional set of six genes without necessarily resorting to an analysis of the entire backcross series. Using this approach, we have defined the linkage of cytochrome b245 beta-chain (Cybb), synapsin (Syn-1), and two members of the X-linked lymphocyte-regulated gene family (Xlr-1, Xlr-2), as well as DXSmh141 and DXSmh172, two loci defined by random genomic probes. All six loci have been localized to the proximal portion of the mouse X chromosome and their order has been defined as Cybb, Otc, Syn-1/Timp, DXSmh141/Xlr-1, DXSmh172, Hprt, Xlr-2, Cf-9. Gene order was established by minimizing multiple recombination events across the region spanning an estimated 20 cM of the proximal X chromosome. The possible significance of the Xlr loci is discussed with respect to other X-chromosome loci that regulate the immune response.

Disciplines

Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy

Keywords

Animals, Blotting, Southern, Chromosome Mapping, Crosses, Genetic, Cytochrome b Group, Female, Genes, Genetic Linkage, Genetic Variation, Male, Mice, Multigene Family, Nerve Tissue Proteins, Recombination, Genetic, Repetitive Sequences, Nucleic Acid, Synapsins, X Chromosome

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