College of Osteopathic Medicine Faculty Articles

PubMed Identifier

2222810

Title

Characterization of the cDNA coding for mouse prothrombin and localization of the gene on mouse chromosome 2.

ISBN or ISSN

1044-5498

Publication Title

DNA and cell biology

Volume

9

Issue

7

Publication Date / Copyright Date

9-1990

First Page

487

Last Page

498

Publisher

Mary Ann Liebert

Abstract

A series of overlapping cDNAs coding for mouse prothrombin (coagulation factor II) have been isolated and the composite DNA sequence has been determined. The complete prothrombin cDNA is 1,987 bp in length [excluding the poly(A) tail] and codes for 18 bp of 5' untranslated sequence, an open reading frame coding for 618 amino acids, a stop codon, and a 3' untranslated region of 112 bp followed by a poly(A) tail. The translated amino acid sequence predicts a molecular weight of 66,087, which includes 10 residues of gamma-carboxyglutamic acid. There are five potential N-linked glycosylation sites. Mouse prothrombin is 81.4% and 77.3% identical to the human and bovine proteins, respectively. Comparison of the cDNA coding for mouse prothrombin to the human and bovine cDNAs indicates 79.9% and 76.5% identity, respectively. Amino acid residues important for the structure and function of human prothrombin are conserved in the mouse and bovine proteins. In the adult mouse and rat, prothrombin is primarily synthesized in the liver, where is constitutes 0.07% of total mRNA as determined by solution hybridization analysis. The genetic locus for mouse prothrombin, Cf-2, has been mapped using an interspecies backcross and DNA fragment differences between the two species. The prothrombin locus lies on mouse chromosome 2, 1.8 +/- 1.3 map units proximal to the catalase locus. The gene order in this region is Cen-Acra-Cf-2-Cas-1-A-Tel. This localization extends the proximal boundary of the known region of homology between mouse chromosome 2 and human chromosome 11p from Cas-1 about 2 map units toward the centromere.

Disciplines

Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy

Keywords

Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Chromosome Mapping, Crosses, Genetic, DNA, Female, Genes, Humans, Male, Mice, Molecular Sequence Data, Open Reading Frames, Prothrombin, Rats, Restriction Mapping, Sequence Homology, Nucleic Acid

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