Faculty Articles

Title

Reaction of pulpal immunoglobulins to oral microorganisms by an enzyme-linked immunosorbent assay

ISBN or ISSN

0099-2399

Publication Title

Journal of Endodontics

Publication Date / Copyright Date

1-1-1987

First Page

260

Last Page

266

Publisher

Elsevier

DOI Number

10.1016/S0099-2399(87)80042-0

Abstract

Dental pulps from teeth clinically diagnosed as having an abnormal response to cold, an abnormal response to cold and lingering pain to heat, or as having responses within normal limits were placed into pulpal explant cultures. The supematant fluids from the cultures were tested by an enzyme-linked immunosorbent assay for immunoglobulins reactive with 16 oral microorganisms implicated in endodontic infections and one nonoral microorganism. Immunoglobulins in each, group were reactive withStreptococcus mutans, Actinomyces naeslundii Lactobacillus casei, Eubacterium alactolyticum, Actinomyces israelli, Peptostreptococcus micros, and Veillonella parvula. Low or negligible reactions were observed with the rest of the bacteria tested. There was a statistically significant (p<0.05) higher level of reactive antibody observed in supermatant fluids of pulpal explant cultures from group cold than those from groups cold-heat and normal for S. mutans and L. casei. These studies demonstrate that immunoglobulins are present in the pulp which react with microorganisms that have been implicated in the carious process. The in vitro pulp explant model utilized may be important in studying the protective or immunopathological role of immunoglobulins in pulpal tissue.

Disciplines

Dentistry

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Peer Reviewed

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