The Frozen Brain Atlas project was conceived by Dr. Robert Speth in the College of Pharmacy at Nova Southeastern University. The creation was shortly followed by the innovation of research assistants Natalie A. Builes (Pharm.D. Candidate) and Dominick J. Casciato (D.P.M. Candidate). Research assistant Samantha T. Bergione joined the team during the development phase and continues to enhance the atlas contents.
The angiotensin receptor autoradiography analysis conducted in the laboratory involves examination of the specialized film containing the desired sectioned mouse brain. Deciphering the exact position of the section within the brain proved time consuming. The Frozen Brain Atlas provides a solution to this problem, which can also be used as a brain mapping technique for educational identification of structure functions. A unique color-coded labeling technique additionally enables the viewer of the atlas to easily identify the basic structures of the mouse brain.
The file available here was made using the Opus Pro software purchased by the NSU Health Professions Division Library.
Natalie Andrea Builes, Dominick Joseph Casciato, Samantha Taylor Bergoine, and Robert Speth
The Frozen Brain Atlas provides students, educators, and scientists with a tool to further explore the architecture of the brain. Current brain atlases allow users to navigate sectioned brain tissue using histological staining; however, they fail to capture naturally visible structures post sectioning. Through immediate post sectioning image collection, the natural contrast of the brain reveals unique structural details that are rarely seen by traditional staining procedures. By utilizing indexing and interactive labeling, the Nova Southeastern University Frozen Brain Atlas will allow identification of brain regions when used as a correlative tool for modalities including receptor autoradiography, immunofluorescence, and other anatomical methods.