Capstone Title

Utilizing Real-Time PCR to Determine Microbial Indicators of Human Pollution: Waste Water Management

Defense Date


Document Type


Degree Name

M.S. Coastal Zone Management

First Advisor

Donald McCorquodale

Second Advisor

George Duncan


The use of qPCR has been implemented in microbiological studies to quantify abundance and expression of taxonomic and functional gene markers that pose contamination threats to drinking, recreational, marine, and fresh water systems. qPCR can offer significant assistance in the field of identifying potential pathogens related to many unknown human illnesses. Within PCR chemistry, utilizing qPCR imparts quantitative and expedient results with greater accuracy to conclude if human pollution is contaminating water sources and to what extent.

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