Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling
We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.
Perry, Richard H.; Bellovin, David I.; Shroff, Emelyn H.; Ismail, Ali I.; Zubuawala, Tahera; Felsher, Dean W.; and Zare, Richard N., "Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling" (2013). Chemistry and Physics Faculty Articles. 190.