Chemistry and Physics Faculty Articles

Title

Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling

Document Type

Article

Publication Date

5-7-2013

Publication Title

Analytical Chemistry

ISSN

0003-2700

Volume

85

Issue/No.

9

First Page

4529

Last Page

4262

Abstract

We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.

Comments

©2013 American Chemical Society

Additional Comments

Air Force Office of Scientific Research grant #: FA 9550-10-1-0235; NIH RO1 grant #: CA 089305, 105102; National Cancer Institute's In-vivo Cellular and Molecular Imaging Center grant #: CA 114747; Integrative Cancer Biology Program grant #: CA 112973; NIH/NCI PO1 grant #: CA034233; Leukemia and Lymphoma Society Translational Research grant #: R6223-07

DOI

10.1021/ac400479j

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Peer Reviewed

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