Biology Faculty Articles

Document Type

Article

Publication Date

9-2019

Keywords

Serine protease inhibitor, serpin, Nec, plant innate immunity, programmed cell death (PCD), cloning, RNAi disease-resistant, transgenic, rice

First Page

1

Last Page

33

Abstract

Programmed Cell Death (PCD) is an important mechanism of plant immune response against diseases. Serine protease inhibitors (‘serpins’) are a conserved superfamily of proteins that inhibit serine protease targets and prevent programmed cell death (PCD) in plants, in absence of pathogen infection. In this project, putative serpin genes in rice (Oryza sativa) which are homologous to necrotic/‘Nec’ serpin genes in Drosophila are identified by bioinformatic analyses. This is followed by cloning of specific exon sequences of the rice serpin genes identified. Finally, one of the cloned sequences is utilized in a series of steps to produce interfering RNA (RNAi), to block the gene expression. Results of this study would help elucidate the importance of serpins in the regulation of PCD in rice. The work could lead to further research aimed at generating disease-resistant transgenic plants.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

ORCID ID

0000-0002-9739-4039

Included in

Biology Commons

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