Title

Clostridium amazonense sp. nov. an obliqately anaerobic bacterium isolated from a remote Amazonian community in Peru

Authors

Lindsey O'Neal, University of Oklahoma
Alexandra J. Obregon-Tito, University of Oklahoma
Raul Y. Tito, University of Oklahoma
Andrew T. Ozga, University of OklahomaFollow
Susan I. Polo, University of Oklahoma
Cecil M. Lewis Jr., University of Oklahoma
Paul Lawson, University of Oklahoma

Document Type

Article

Publication Date

10-2015

Publication Title

Anaerobe

Keywords

Clostridium amazonense sp. nov., Indigenous community, 16S rRNA phylogeny, Taxonomy

ISSN

1075-9964

Volume

35

Issue/No.

Part B

First Page

33

Last Page

37

Abstract

A strictly anaerobic Gram-stain positive, spore-forming, rod-shaped bacterium designated NE08V^T, was isolated from a fecal sample of an individual residing in a remote Amazonian community in Peru. Phylogenetic analysis based on the 16S rRNA gene sequence showed the organism belonged to the genus Clostridium and is most closely related to Clostridium vulturis (97.4% sequence similarity) and was further characterized using biochemical and chemotaxonomic methods. The major cellular fatty acids were anteiso C_13:0 and C_16:0 with a genomic DNA G + C content of 31.6 mol%. Fermentation products during growth with PYG were acetate and butyrate. Based on phylogenetic, phenotypic and chemotaxonomic information, strain NE08V was identified as representing a novel species of the genus Clostridium, for which the name Clostridium amazonense sp. nov. is proposed. The type strain is NE08V^T (DSM 23598^T = CCUG 59712^T).

Comments

The 16S rRNA sequence of strain NE08V^T (DSM 23598^T = CCUG 59712^T) have been deposited in GenBank under accession number KP281434.

NSUWorks Citation

O'Neal, Lindsey; Alexandra J. Obregon-Tito; Raul Y. Tito; Andrew T. Ozga; Susan I. Polo; Cecil M. Lewis Jr.; and Paul Lawson. 2015. "Clostridium amazonense sp. nov. an obliqately anaerobic bacterium isolated from a remote Amazonian community in Peru." Anaerobe 35, (Part B): 33-37. doi:10.1016/j.anaerobe.2015.06.005.

ORCID ID

0000-0003-4540-7106

ResearcherID

D-1147-2018

DOI

10.1016/j.anaerobe.2015.06.005