Bioinspired materials, Differential extraction, DNA casework backlog, Forensic cases, Microfluids
One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time‐consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60–90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl‐LewisX), a major carbohydrate ligand for sperm‐egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70–92% sperm capture efficiency and a 60–92% reduction in epithelial fraction. Captured sperm are then lysed on‐chip and sperm DNA is isolated. This method reduces assay‐time from 8 h to 80 min, providing an inexpensive alternative to current differential extraction techniques, accelerating identification of suspects and advancing public safety.
Inci, Fatih; Mehmet O. Ozen; Yeseren Saylan; Morteza Miansari; Duygu Cimen; Raghu Dhara; Thiruppathiraja Chinnasamy; Mehmet Yuksekkaya; Chiara Filippini; Deepan Kishore Kumar; Semih Calamak; Yusuf Yesil; Naside Gozde Durmus; George Duncan; Leonard Klevan; and Utkan Demirci. 2018. "A Novel On‐Chip Method for Differential Extraction of Sperm in Forensic Cases." Advanced Science 2018, (1800121): 1-12. doi:10.1002/advs.201800121.