Title

Genetic Factors Leading to Chronic Epstein–Barr Virus Infection and Nasopharyngeal Carcinoma in South East China: Study Design, Methods and Feasibility

Authors

Xiu Chan Guo, National Cancer Institute at Frederick; Cangwu Institute for Nasopharyngeal Carcinoma Control and Prevention - China
Kevin Scott, National Cancer Institute at Frederick
Yan Liu, National Cancer Institute at Frederick
Michael Dean, National Cancer Institute at Frederick
Victor David, National Cancer Institute at Frederick
George W. Nelson, National Cancer Institute at Frederick
Randall C. Johnson, National Cancer Institute at Frederick
Holli H. Dilks, National Cancer Institute at Frederick
J. A. Lautenberger, National Cancer Institute at Frederick
Bailey Kessing, National Cancer Institute at Frederick
Janice S. Martenson, National Cancer Institute at Frederick
Li Guan, National Cancer Institute at Frederick
Shan Sun, National Cancer Institute at Frederick
Hong Deng, Cancer Institute of Wuzhou - China
Yuming Zheng, Cancer Institute of Wuzhou - China
Guy de The, Centre National de la Recherche Scientifique
Jian Liao, Cangwu Institute for Nasopharyngeal Carcinoma Control and Prevention - China
Yi Zeng, Cangwu Institute for Nasopharyngeal Carcinoma Control and Prevention - China
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
Cheryl Winkler, National Cancer Institute at Frederick

Document Type

Article

Publication Date

6-2006

Publication Title

Human Genomics

Keywords

Nasopharyngeal carcinoma, Chromosome 4, Microsatellite, Association study, Epstein-Barr virus

ISSN

1473-9542

Volume

2

Issue/No.

6

First Page

365

Last Page

375

Abstract

Nasopharyngeal carcinoma (NPC) is a complex disease caused by a combination of Epstein-Barr virus chronic infection, the environment and host genes in a multi-step process of carcinogenesis. The identity of genetic factors involved in the development of chronic Epstein-Barr virus infection and NPC remains elusive, however. Here, we describe a two-phase, population-based, case-control study of Han Chinese from Guangxi province, where the NPC incidence rate rises to a high of 25-50 per 100,000 individuals. Phase I, powered to detect single gene associations, enrolled 984 subjects to determine feasibility, to develop infrastructure and logistics and to determine error rates in sample handling. A microsatellite screen of Phase I study participants, genotyped for 319 alleles from 34 microsatellites spanning an 18-megabase region of chromosome 4 (4p15.1-q12), previously implicated by a linkage analysis of familial NPC, found 14 alleles marginally associated with developing NPC or chronic immunoglobulin A production (p = 0.001-0.03). These associations lost significance after applying a correction for multiple tests. Although the present results await confirmation, the Phase II study population has tripled patient enrolment and has included environmental covariates, offering the potential to validate this and other genomic regions that influence the onset of NPC.

Comments

©Henry Stewart Publications 2006

Additional Comments

National Cancer Institute contract #: N01-CO-12400

NSUWorks Citation

Guo, Xiu Chan; Kevin Scott; Yan Liu; Michael Dean; Victor David; George W. Nelson; Randall C. Johnson; Holli H. Dilks; J. A. Lautenberger; Bailey Kessing; Janice S. Martenson; Li Guan; Shan Sun; Hong Deng; Yuming Zheng; Guy de The; Jian Liao; Yi Zeng; Stephen J. O'Brien; and Cheryl Winkler. 2006. "Genetic Factors Leading to Chronic Epstein–Barr Virus Infection and Nasopharyngeal Carcinoma in South East China: Study Design, Methods and Feasibility." Human Genomics 2, (6): 365-375. https://nsuworks.nova.edu/cnso_bio_facarticles/788

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015