Title

Automated Single-Strand Conformation Polymorphism Reveals Low Diversity of a Major Histocompatibility Complex Class II Gene in the Threatened New Zealand Sea Lion

Authors

G. M. Lento, University of Auckland
C. S. Baker, University of Auckland
Victor David, National Cancer Institute at Frederick
Naoya Yuhki, National Cancer Institute at Frederick
N. J. Gales, Australian Antarctic Division
Stephen J. O'Brien, National Cancer Institute at FrederickFollow

Document Type

Article

Publication Date

9-2003

Publication Title

Molecular Ecology Resources

Keywords

ABI, DQB, Genetic diversity, Major Histocompatibility Complex, New Zealand sea lion, Population genetics, SSCP

ISSN

1755-098X

Volume

3

Issue/No.

2

First Page

346

Last Page

349

Abstract

Single-strand conformation polymorphism (SSCP) analysis of the second exon of the DQB gene in the endemic New Zealand sea lion (Phocarctos hookeri) was automated using fluorescently-labelled PCR primers and a capillary sequencer. SSCP mobility profiles (n = 61 individuals) were confirmed by direct sequencing of genomic polymerase chain reaction (PCR) products (n = 39). We found only two alleles defined by changes at a single codon of this apparently functional locus. SSCP at a constant temperature above ambient allowed consistent and rapid genotyping. By comparison, sequence-based genotyping was highly dependent on the threshold used to identify secondary peaks indicative of a heterozygote.

Comments

©2003 Blackwell Publishing Ltd

NSUWorks Citation

Lento, G. M.; C. S. Baker; Victor David; Naoya Yuhki; N. J. Gales; and Stephen J. O'Brien. 2003. "Automated Single-Strand Conformation Polymorphism Reveals Low Diversity of a Major Histocompatibility Complex Class II Gene in the Threatened New Zealand Sea Lion." Molecular Ecology Resources 3, (2): 346-349. https://nsuworks.nova.edu/cnso_bio_facarticles/602

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015