Influence of Temperature and Gas Atmosphere on In-Vitro Fertilization and Embryo Development in Domestic Cats
Journal of Reproduction and Fertility
Cat, Fertilization, Embryo, In vitro culture, Incubation, Temperature, Gas atmosphere
The influence of culture temperature and gas atmosphere on in-vitro fertilization and embryo development was examined in the domestic cat. In Exp. 1, eggs were fertilized and cultured in 5% CO2 in air at 37, 38 or 39°C. Experiment 2 evaluated the effects of 5% CO2 in air; 5% CO2, 5% O2 and 90% N2; and 10% CO2 in air. Fertilization (cleavage) and development to the morula/blastocyst stage were not influenced (P > 0·05) by variations in temperature and gas composition. Despite changing these culture conditions, egg cleavage averaged ∼75% and >80% of the 2-cell embryos proceeded to morulae in vitro. However, the partial in-vitro morula-to-blastocyst developmental block normally observed in this species was not removed.
Johnston, L. A.; A. M. Donoghue; Stephen J. O'Brien; and David E. Wildt. 1991. "Influence of Temperature and Gas Atmosphere on In-Vitro Fertilization and Embryo Development in Domestic Cats." Journal of Reproduction and Fertility 92, (2): 377-382. https://nsuworks.nova.edu/cnso_bio_facarticles/330