Title

The Human Chromogranin A Gene: Chromosome Assignment and RFLP Analysis.

Authors

William Modi, National Cancer Institute at Frederick
Michael A. Levine, Johns Hopkins University
Hector Seuanez, National Cancer Institute at Frederick
Michael Dean, National Cancer Institute at Frederick
Stephen J. O'Brien, National Cancer Institute at FrederickFollow

Document Type

Article

Publication Date

11-1989

Publication Title

American Journal of Human Genetics

ISSN

0002-9297

Volume

45

Issue/No.

5

First Page

814

Last Page

818

Abstract

Chromogranin A/secretory protein I (CgA) is a glycoprotein that is stored and released along with peptide hormones and neurotransmitters from several tissues, although its exact function is not known. A cDNA (gene symbol CHGA) clone was used as a probe in Southern blot analyses of human-rodent somatic cell hybrid DNAs. Discordancy analysis allowed confirmation of the assignment of the gene to chromosome 14. These results were extended using in situ chromosome hybridization, and a signal was found at 14q32. BglII digestion of genomic DNA from 28 unrelated Caucasian individuals probed with CHGA detected a two-allele RFLP with allelic frequencies of .34 and .66.

Comments

This material is in the public domain, and no copyright is claimed.

Additional Comments

NIH grant #: Dk-38990; Health and Human Services contract #: NO1-CO-74102

NSUWorks Citation

Modi, William; Michael A. Levine; Hector Seuanez; Michael Dean; and Stephen J. O'Brien. 1989. "The Human Chromogranin A Gene: Chromosome Assignment and RFLP Analysis.." American Journal of Human Genetics 45, (5): 814-818. https://nsuworks.nova.edu/cnso_bio_facarticles/302

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015