Title

A New Moderately Repetitive DNA Sequence Family of Novel Organization

Authors

Neal D. Epstein, National Heart, Lung, and Blood Institute
S. Karlsson, National Heart, Lung, and Blood Institute
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
William S. Modi, National Cancer Institute at Frederick
A. Moulton, National Heart, Lung, and Blood Institute
Arthur W. Nienhuis, National Heart, Lung, and Blood Institute

Document Type

Article

Publication Date

3-15-1987

Publication Title

Nucleic Acids Research

ISSN

0305-1048

Volume

15

Issue/No.

5

First Page

2327

Last Page

2341

Abstract

In cloning adenovirus homologous sequences, from a human cosmid library, we identified a moderately repetitive DNA sequence family consisting of tandem arrays of 2.5 kb members. A member was sequenced and several non-adjacent, 15–20 bp G-C rich segments with homology to the left side of adenovirus were discovered. The copy number of 400 members is highly conserved among humans. Southern blots of partial digests of human DNA have verified the tandem array of the sequence family. The chromosomal location was defined by somatic cell genetics and in situ hybridization. Tandem arrays are found only on chromosomes 4 (4q31) and 19 (ql3.l-ql3.3). Homologous repetitive sequences are found in DNA of other primates but not in cat or mouse. Thus we have identified a new family of moderately repetitive DNA sequences, unique because of its organization in clustered tandem arrays, its length, its chromosomal location, and its lack of homology to other moderately repetitive sequence families.

Comments

©IRL Press Limited, Oxford, England

NSUWorks Citation

Epstein, Neal D.; S. Karlsson; Stephen J. O'Brien; William S. Modi; A. Moulton; and Arthur W. Nienhuis. 1987. "A New Moderately Repetitive DNA Sequence Family of Novel Organization." Nucleic Acids Research 15, (5): 2327-2341. https://nsuworks.nova.edu/cnso_bio_facarticles/154

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015