Wastewater was screened for the presence of functionally significant mutations in SARS-CoV-2 associated with emerging variants of concern (VOC) by ddPCR, and results accorded with sequencing of clinical samples from the same region. We propose that PCR-based screening of wastewater can provide a powerful tool for rapid and inexpensive screening of large population segments for VOC-associated mutations and can hone complementary sampling and sequencing of direct (human) test material to track emerging VOC.
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Gering, Eben; Jacob Colbert; Sarah Schmedes; George Duncan; Jose V. Lopez; Jessy Motes; James Weiss; Taj Azarian; Omer Tekin; and Jason Blanton. 2021. "ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater." medRxiv , (): 21255119. doi:10.1101/2021.04.08.21255119.
0000-0002-4931-5316, 0000-0002-4931-5316, 0000-0002-1637-4125