Title

ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater

Authors

Eben Gering, Nova Southeastern UniversityFollow
Jacob Colbert, Nova Southeastern University
Sarah Schmedes, Florida Department of Health
George Duncan, Nova Southeastern UniversityFollow
Jose V. Lopez, Nova Southeastern UniversityFollow
Jessy Motes, Florida Department of Health
James Weiss, Florida Department of Health
Taj Azarian, Florida Department of Health
Omer Tekin, Florida Department of Health
Jason Blanton, Florida Department of Health

Document Type

Article

Publication Date

4-13-2021

Publication Title

medRxiv

First Page

21255119

Abstract

Wastewater was screened for the presence of functionally significant mutations in SARS-CoV-2 associated with emerging variants of concern (VOC) by ddPCR, and results accorded with sequencing of clinical samples from the same region. We propose that PCR-based screening of wastewater can provide a powerful tool for rapid and inexpensive screening of large population segments for VOC-associated mutations and can hone complementary sampling and sequencing of direct (human) test material to track emerging VOC.

Comments

Funding was provided via a CDC epidemiology and laboratory capacity grant (ELC).

Additional Comments

The copyright holder for this preprint is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

NSUWorks Citation

Gering, Eben; Jacob Colbert; Sarah Schmedes; George Duncan; Jose V. Lopez; Jessy Motes; James Weiss; Taj Azarian; Omer Tekin; and Jason Blanton. 2021. "ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater." medRxiv , (): 21255119. doi:10.1101/2021.04.08.21255119.

ORCID ID

0000-0002-4931-5316, 0000-0002-4931-5316, 0000-0002-1637-4125

ResearcherID

J-8619-2012, F-8809-2011

DOI

10.1101/2021.04.08.21255119