Theses and Dissertations

Copyright Statement

All rights reserved. This publication is intended for use solely by faculty, students, and staff of Nova Southeastern University. No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, now known or later developed, including but not limited to photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author or the publisher.

Defense Date

1995

Document Type

Thesis - NSU Access Only

Degree Name

M.S. Marine Biology

Second Degree Name

M.S. Coastal Zone Management

Department

Oceanographic Center

First Advisor

Richard Spieler

Second Advisor

Richard E. Dodge

Third Advisor

Robert Hueter

Fourth Advisor

Charles Manire

Abstract

Previous research has indicated species-specific stress responses in sharks. To assess the biochemical effects of net capture and restraint, nineteen serum constituents were measured in three species of sharks: bonnethead, Sphyrna tiburo (Linnaeus, 1758), n = 36; blacktip, Carcharhinus limbatus (Muller and Henle, 1841), n = 33; and bull, C. leucas (Muller and Henle, 1841), n = 27. Sharks were captured in gill nets placed at various locations along the southwest coast of Florida between April and July, 1994. Stress level of each animal was judged in five categories using an index of behavioral response to capture and restraint devised for use in tag-recapture studies. These categories ranged from level I "minimal stress response" to level 5 "moribund or dead". The serum constituents assayed included: glucose, creatinine, uric acid, sodium, chloride, potassium, inorganic phosphorus, total and ionized calcium, total protein, albumin, globulin, alkaline phosphatase, lactate, lactate dehydrogenase; aspartate aminotransferase, triglycerides, cholesterol, and total iron. Hematocrit was also measured for each sample.

There were significant intraspecific differences in several serum constituent values for all three species. With increased stress, S. tiburo had increased potassium, inorganic phosphorus, uric acid, alkaline phosphatase and lactate and decreased glucose levels (P < 0.05 ANOVA or Kruskal-Wallis). C. limbatus had increased uric acid, potassium, lactate, total and ionized calcium, inorganic phosphorus and alkaline phosphatase (P < 0.05), while C. leucas had increased potassium, inorganic phosphorus, aspartate aminotransferase, lactate and decreased glucose levels (P < 0.05). The wide variation observed in serum constituent values among species when compared with intraspecific differences in lifestyle, behavior, general morphology and environmental requirements may help explain differences in species-specific mortality rates previously documented.

Files over 10MB may be slow to open. For best results, right-click and select "Save as..."

To access this thesis/dissertation you must have a valid nova.edu OR mynsu.nova.edu email address and create an account for NSUWorks.

Free My Thesis

If you are the author of this work and would like to grant permission to make it openly accessible to all, please click the Free My Thesis button.

  Link to NovaCat

Share

COinS