Title

Complete Nucleotide Sequences of the Domestic Cat (Felis catus) Mitochondrial Genome and a Transposed mtDNA Tandem Repeat (Numt) in the Nuclear Genome

Document Type

Article

Publication Date

4-1996

Publication Title

Genomics

Keywords

Amino acid sequence, Animals, Base sequence, Cats/genetics, Cell nucleus, DNA transposable elements, Mitochondrial DNA, Genome, Humans, Mitochondria, Molecular sequence data, Nucleic acid conformation, Phylogeny, Repetitive sequences, Nucleic acid

ISSN

0888-7543

Volume

33

Issue/No.

2

First Page

229

Last Page

246

Abstract

The complete 17,009-bp mitochondrial genome of the domestic cat, Felis catus, has been sequenced and conforms largely to the typical organization of previously characterized mammalian mtDNAs. Codon usage and base composition also followed canonical vertebrate patterns, except for an unusual ATC (non-AUG) codon initiating the NADH dehydrogenase subunit 2 (ND2) gene. Two distinct repetitive motifs at opposite ends of the control region contribute to the relatively large size (1559 bp) of this carnivore mtDNA. Alignment of the feline mtDNA genome to a homologous 7946-bp nuclear mtDNA tandem repeat DNA sequence in the cat, Numt, indicates simple repeat motifs associated with insertion/deletion mutations. Overall DNA sequence divergence between Numt and cytoplasmic mtDNA sequence was only 5.1%. Substitutions predominate at the third codon position of homologous feline protein genes. Phylogenetic analysis of mitochondrial gene sequences confirms the recent transfer of the cytoplasmic mtDNA sequences to the domestic cat nucleus and recapitulates evolutionary relationships between mammal species.

Comments

©1996 Academic Press. All rights reserved.

ORCID ID

0000-0002-1637-4125; 0000-0001-7353-8301

ResearcherID

F-8809-2011; N-1726-2015

DOI

10.1006/geno.1996.0188