Title

Novel flow-cytometric method for separating cell types in differentiated F9 embryoid bodies

ISBN or ISSN

1552-4930

Publication Title

Cytometry

Volume

21

Issue

2

Publication Date / Copyright Date

10-1-1995

First Page

145

Last Page

152

Publisher

Wiley-Liss

DOI Number

10.1002/cyto.990210206

Abstract

The differentiation of F9 teratocarcinoma cells mimics the formation of a mouse embryonic tissue, the primitive endoderm. In vitro, small aggregates of F9 cells, termed embryoid bodies, differentiate in response to retinoic acid and develop a surface epithelium that is characterized by the production of alpha-fetoprotein. In the present study, cellular autofluorescence profiles obtained by fluorescence-activated embryoid bodies were composed of a single type of cell. In contrast, retinoic acid-induced embryoid bodies were composed of two cell types: a major population displaying autofluorescence levels similar to those of cells from undifferentiated embryoid bodies and a second population displaying higher autofluorescence. RNA analyses demonstrated that the transcription of alpha-fetoprotein was associated only with the more highly autofluorescent population, indicating that flow cytometry provides a novel mechanism for the separation of undifferentiated cells from differentiated endoderm cells in F9 embryoid bodies.

Disciplines

Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences

Keywords

Animals, Antibodies, Cell Differentiation, Cell Line, Cell Separation, Flow Cytometry, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Immunohistochemistry, Mice, RNA, RNA, Neoplasm, Rabbits, Teratocarcinoma, Tretinoin, Tumor Cells, Cultured, Wheat Germ Agglutinins

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