College of Osteopathic Medicine Faculty Articles

Title

Identification of an X-linked Member of the Odc Gene Family in the Mouse

ISBN or ISSN

0305-1048

Publication Title

Nucleic Acids Research

Volume

16

Issue

4

Publication Date / Copyright Date

2-1-1988

DOI Number

10.1093/nar/16.4.1642

Abstract

The mouse ornithine decarboxylase gene is part of a dispersed family of at least 14 related loci. Eight Odc loci have been assigned to 7 different autosomes, although the structural locus has not been identified. Recent mapping of the mouse genome has exploited the sequence diversity apparent between inbred strains and feral species, notably Mus spretus. This diversity, expressed as restriction fragment length polymorphism, together with the unique segregation pattern of X-linked loci, has facilitated the assignment of molecular markers to the mouse X chromosome. Among F1 progeny from reciprocal crosses between C57BL/6JRos and wild-derived M. musculus from Denmark a single 2.7 kb Odc band can be distinguished as X-linked from multiple autosomal bands. This fragment specifically defines a locus on the M. musculus Denmark X chromosome which is not inherited in the progeny of the Denmark sire. A corresponding 7.0 kb X-linked fragment in C57BL/6JRos has been identified in a cross with M. spretus which is obscured in the Denmark cross with autosomal bands. Although less divergent evolutionarily than M. spretus, the M. musculus stock remains a potent source of restriction variation. Unlike M. spretus, the use of M. musculus as the wild-derived parent allows for reciprocal F1 crosses, so that unique X-linked fragments originating from either the wild or inbred genome can be discerned.

Disciplines

Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy

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