Biology Faculty Proceedings, Presentations, Speeches, Lectures

Novel Pluripotent Adult Stem Cell Source for Neurogenesis

Event Name/Location

Biomedical Engineering Conference (SBEC), 2013 29th Southern

Presntation Date

5-3-2013

Document Type

Conference Proceeding

Proceeding Title

SBEC 2013: 29th Southern Biomedical Engineering Conference

ISBN

978-0-7695-5032-9

Keywords

Stem cells, Gene expression, Ligaments, Immune System, Sociology, Statistics, Media, Patient Treatment, Biochemistry, Cellular Biophysics, Genetics, Neurophysiology, time 6 day, pluripotent adult stem cell source, periodontal ligament stem cells, regenerative medicine, in-vivo germ layers, in-vitro neurogenesis, neuroinduced Connexin 43+ PDLSC, neurogenic cocktail media, cell morphology, gene expression, immunohistochemistry, two-tailed t-test, neuro filament medium, neural lineage

Description

A novel pluripotent stem cell population discovered by our laboratory, periodontal ligament stem cells (PDLSCs) are a viable source for regenerative medicine. We have demonstrated that these cells form all three germ layers in-vivo, and hypothesized that PDLSCs would be an ideal cell source for neurogenesis in-vitro. In our study, we evaluate the potential of Connexin 43+ PDLSCs to differentiate into a neural lineage. Control Connexin 43+ PDLSCs were cultured in complete culture media. Neuro-induced Connexin 43+PDLSCs were grown in a neurogenic cocktail media. Media was changed every three days for a total of 6 days of treatment. Following treatment, cell morphology, gene expression, and immunohistochemistry were evaluated. Gene expression data was analyzed using a two-tailed t-test, with p<;0.05 considered significant. Morphological differences were evident in neuro-induced Connexin 43+ PDLSCs, along with a significant increase in gene expression of neuro filament medium over control samples. Immunohistochemical staining in neuro-induced Connexin 43+ PDLSCs demonstrated neurite-like processes and synaptophysin not found in control samples. Conclusion: Our neuro-induction protocol successfully induces Connexin 43+ PDLSCs to a neural lineage.

DOI

10.1109/SBEC.2013.16

Publisher

IEEE

First Page

15

Last Page

16

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