Novel Pluripotent Adult Stem Cell Source for Neurogenesis
Biomedical Engineering Conference (SBEC), 2013 29th Southern
Stem cells, Gene expression, Ligaments, Immune System, Sociology, Statistics, Media, Patient Treatment, Biochemistry, Cellular Biophysics, Genetics, Neurophysiology, time 6 day, pluripotent adult stem cell source, periodontal ligament stem cells, regenerative medicine, in-vivo germ layers, in-vitro neurogenesis, neuroinduced Connexin 43+ PDLSC, neurogenic cocktail media, cell morphology, gene expression, immunohistochemistry, two-tailed t-test, neuro filament medium, neural lineage
A novel pluripotent stem cell population discovered by our laboratory, periodontal ligament stem cells (PDLSCs) are a viable source for regenerative medicine. We have demonstrated that these cells form all three germ layers in-vivo, and hypothesized that PDLSCs would be an ideal cell source for neurogenesis in-vitro. In our study, we evaluate the potential of Connexin 43+ PDLSCs to differentiate into a neural lineage. Control Connexin 43+ PDLSCs were cultured in complete culture media. Neuro-induced Connexin 43+PDLSCs were grown in a neurogenic cocktail media. Media was changed every three days for a total of 6 days of treatment. Following treatment, cell morphology, gene expression, and immunohistochemistry were evaluated. Gene expression data was analyzed using a two-tailed t-test, with p<;0.05 considered significant. Morphological differences were evident in neuro-induced Connexin 43+ PDLSCs, along with a significant increase in gene expression of neuro filament medium over control samples. Immunohistochemical staining in neuro-induced Connexin 43+ PDLSCs demonstrated neurite-like processes and synaptophysin not found in control samples. Conclusion: Our neuro-induction protocol successfully induces Connexin 43+ PDLSCs to a neural lineage.
Conference Proceeding Title
SBEC 2013: 29th Southern Biomedical Engineering Conference
Fortino, Veronica R.; Pawley, Devon; Pelaez, Daniel; and Cheung, Herman S., "Novel Pluripotent Adult Stem Cell Source for Neurogenesis" (2013). Biology Faculty Proceedings, Presentations, Speeches, Lectures. 299.