Biology Faculty Articles

Title

Cloning and Mapping of Cat (Felis catus) Immunoglobulin and T-Cell Receptor Genes

Document Type

Article

Publication Date

1-1998

Publication Title

Immunogenetics

Keywords

Cat, Ig, TcR, Cloning, Mapping

ISSN

0093-7711

Volume

47

Issue/No.

3

First Page

226

Last Page

233

Abstract

Molecular cloning and chromosomal mapping of the cat immunoglobulin (Ig) and T-cell receptor (TcR) genes were carried out to provide basic information for genetic analysis of immunologic diseases including leukemias and lymphomas in cats. We cloned two Ig constant genes, IGHM and IGHG and three TcR constant genes, TRAC, TRGC, and TRDC, by polymerase chain reaction (PCR) amplification of cDNA from cat peripheral blood mononuclear cells. For chromosomal mapping of the Ig and TcR loci including the IGK, IGL, and TRB on the cat genome, we performed PCR screening of DNAs from 37 cat × rodent somatic cell hybrids by using specific primers for the given genes. Consequently, three loci for IGH, TRA, and TRD, and two loci for TRB and TRG were found to be syntenic and assigned to cat chromosomes (FCA) B3 and A2, respectively. Further, IGK and IGL loci were mapped on FCA A3 and D3, respectively. These findings support the notion that the genetic linkages between the Ig and TcR genes are extensively conserved between humans and cats.

Comments

©Springer-Verlag 1998

Additional Comments

GenBank accession #s: D89018, D89020-D89025

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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