Title

Immunologic Profile of Highly Exposed Yet HIV Type 1-Seronegative Men

Authors

Otto Yang, University of California - Los Angeles
W. John Boscardin, University of California - Los Angeles
Jose Matud, University of California - Los Angeles
Mary Ann Hausner, University of California - Los Angeles
Lance E. Hultin, University of California - Los Angeles
Patricia M. Hultin, University of California - Los Angeles
Roger Shih, University of California - Los Angeles
John Ferbas, University of California - Los Angeles
Frederick P. Siegal, Saint Vincent Catholic Medical Center
Michael Shodell, Saint Vincent Catholic Medical Center
Gene M. Shearer, National Cancer Institute at Bethesda
Edith Grene, National Cancer Institute at Bethesda
Mary Carrington, National Cancer Institute at Frederick
Stephen J. O'Brien, National Cancer Institute at FrederickFollow
Charles B. Price, University of California - Los Angeles
Roger Detels, University of California - Los Angeles
Beth D. Jamieson, University of California - Los Angeles
Janis Giorgi, University of California at Los Angeles

Document Type

Article

Publication Date

9-2002

Publication Title

AIDS Research and Human Retroviruses

ISSN

0889-2229

Volume

18

Issue/No.

14

First Page

1051

Last Page

1065

Abstract

The host immune factors that determine susceptibility to HIV-1 infection are poorly understood. We compared multiple immunologic parameters in three groups of HIV-1-seronegative men: 14 highly exposed (HR10), 7 previously reported possibly to have sustained transient infection (PTI), and a control group of 14 low risk blood bank donors (BB). Virus-specific cellular immune assays were performed for CD4⁺ T helper cell responses, CD8⁺cytotoxic T lymphocyte activity, CD8⁺ cell chemokine release, and CD8⁺ cell-derived antiviral soluble factor activity. General immune parameters evaluated included CCR5 genotype and phenotype, interferon α production by PBMCs, leukocyte subset analysis, and detailed T lymphocyte phenotyping. Comparisons revealed no detectable group-specific differences in measures of virus-specific immunity. However, the HR10 group differed from the BB group in several general immune parameters, having higher absolute monocyte counts, higher absolute CD8⁺ T cell counts and percentages, lower naive and higher terminal effector CD8⁺ cells, and lower levels of CD28⁺CD8⁺cells. These changes were not associated with seropositivity for other chronic viral infections. The PTI men appeared to have normal levels of monocytes and slightly elevated levels of CD8⁺ T cells (also with increased effector and decreased naive cells). Although we cannot entirely exclude the contribution of other chronic viral infections, these findings suggest that long-lived systemic cellular antiviral immunity as detected by our assays is not a common mechanism for resistance to infection, and that resistance may be multifactorial. General immune parameters reflected by CD8⁺ T cell levels and activation, and monocyte concentrations may affect the risk of infection with HIV-1, and/or serve as markers of exposure.

Comments

©2002 Mary Ann Liebert, Inc.

NSUWorks Citation

Yang, Otto; W. John Boscardin; Jose Matud; Mary Ann Hausner; Lance E. Hultin; Patricia M. Hultin; Roger Shih; John Ferbas; Frederick P. Siegal; Michael Shodell; Gene M. Shearer; Edith Grene; Mary Carrington; Stephen J. O'Brien; Charles B. Price; Roger Detels; Beth D. Jamieson; and Janis Giorgi. 2002. "Immunologic Profile of Highly Exposed Yet HIV Type 1-Seronegative Men." AIDS Research and Human Retroviruses 18, (14): 1051-1065. https://nsuworks.nova.edu/cnso_bio_facarticles/611

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015