Biology Faculty Articles

Title

The Feline Major Histocompatibility Complex is Rearranged by an Inversion with a Breakpoint in the Distal Class I Region

Document Type

Article

Publication Date

1-2005

Publication Title

Immunogenetics

ISSN

0093-7711

Volume

56

Issue/No.

10

First Page

702

Last Page

709

Abstract

In order to determine the genomic organization of the major histocompatibility complex (MHC) of the domestic cat (Felis catus), DNA probes for 61 markers were designed from human MHC reference sequences and used to construct feline MHC BAC contig map spanning ARE1 in the class II region to the olfactory receptor complex in the extended class I region. Selected BAC clones were then used to identify feline-specific probes for the three regions of the mammalian MHC (class II–class III–class I) for radiation hybrid mapping and fluorescent in situ hybridization to refine the organization of the domestic cat MHC. The results not only confirmed that the p-arm of domestic cat B2 is inverted relative to human Chromosome 6, but also demonstrated that one inversion breakpoint localized to the distal segment of the MHC class I between TRIM39 and TRIM26. The inversion thus disjoined the ~2.85 Mb of MHC containing class II–class III–class I (proximal region) from the ~0.50 Mb of MHC class I/extended class I region, such that TRIM39 is adjacent to the Chromosome B2 centromere and TRIM26 is adjacent to the B2 telomere in the domestic cat.

Comments

©Springer-Verlag 2004

Additional Comments

National Institute of Health contract #: N01-C0-12400

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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