Development and Characterization of Microsatellite Loci in the Endangered Oyster Mussel Epioblasma capsaeformis (Bivalvia: Unionidae)
Molecular Ecology Notes
DNA, Epioblasma, Freshwater mussel, Microsatellites
Primers for 10 polymorphic microsatellite loci were developed and characterized for the endangered oyster mussel Epioblasma capsaeformis from the Clinch River, Tennessee. Microsatellite loci also were tested in four other populations or species. Amplification was successful for most loci in these closely related endangered species or populations; therefore, a high level of flanking sequence similarity was inferred for this group of species and populations. Allelic diversity ranged from nine to 20 alleles/locus, and averaged 13.6/locus. This study demonstrated the feasibility of using polymerase chain reaction (PCR) primers to amplify microsatellite loci across freshwater mussel species to conduct population genetics studies.
Jones, Jess W.; Melanie Culver; Victor David; Jennifer Struthers; Nathan A. Johnson; Richard J. Neves; Stephen J. O'Brien; and Eric M. Hallerman. 2004. "Development and Characterization of Microsatellite Loci in the Endangered Oyster Mussel Epioblasma capsaeformis (Bivalvia: Unionidae)." Molecular Ecology Notes 4, (4): 649-652. http://nsuworks.nova.edu/cnso_bio_facarticles/543