Title

Development of Y Chromosome Intraspecific Polymorphic Markers in the Felidae

Authors

Shu-Jin Luo, National Cancer Institute at Frederick; University of Minnesota - St. Paul
Warren E. Johnson, National Cancer Institute at Frederick
Victor A. David, National Cancer Institute at Frederick
Marilyn Menotti-Raymond, National Cancer Institute at Frederick
R. Stanyon, University of Florence - Italy
Qing-xiu Cai, Beijing Normal University - China
Thomas W. Beck, National Cancer Institute at Frederick
Naoya Yuhki, National Cancer Institute at Frederick
Jill Pecon-Slattery, National Cancer Institute at Frederick
James L. D. Smith, University of Minnesota- St. Paul
Stephen J. O'Brien, National Cancer Institute at FrederickFollow

Document Type

Article

Publication Date

7-2007

Publication Title

Journal of Heredity

ISSN

0022-1503

Volume

98

Issue/No.

5

First Page

400

Last Page

413

Abstract

Y chromosome haplotyping based on microsatellites and single nucleotide polymorphisms (SNPs) has proved to be a powerful tool for population genetic studies of humans. However, the promise of the approach is hampered in the majority of nonhuman mammals by the lack of Y-specific polymorphic markers. We were able to identify new male-specific polymorphisms in the domestic cat Felis catus and 6 additional Felidae species with a combination of molecular genetic and cytogenetic approaches including 1) identifying domestic cat male-specific microsatellites from markers generated from a male cat microsatellite-enriched genomic library, a flow-sorted Y cosmid library, or a Y-specific cat bacteria artificial chromosome (BAC) clone, (2) constructing microsatellite-enriched libraries from flow-sorted Y chromosomes isolated directly from focal wildcat species, and (3) screening Y chromosome conserved anchored tagged sequences primers in Felidae species. Forty-one male-specific microsatellites were identified, but only 6 were single-copy loci, consistent with the repetitive nature of the Y chromosome. Nucleotide diversity (π) of Y-linked intron sequences (2.1 kbp) was in the range of 0 (tiger) to 9.95 × 10⁻⁴ (marbled cat), and the number of SNPs ranged from none in the tiger to 7 in the Asian leopard cat. The Y haplotyping system described here, consisting of 4 introns (SMCY3,SMCY7, UTY11, and DBY7) and 1 polymorphic microsatellite (SMCY-STR), represents the first available markers for tracking intraspecific male lineage polymorphisms in Felidae species and promises to provide significant insights to evolutionary and population genetic studies of the species.

Comments

©The American Genetic Association. 2007. All rights reserved.

Additional Comments

National Cancer Institute contract #: N01-CO-12400

NSUWorks Citation

Luo, Shu-Jin; Warren E. Johnson; Victor A. David; Marilyn Menotti-Raymond; R. Stanyon; Qing-xiu Cai; Thomas W. Beck; Naoya Yuhki; Jill Pecon-Slattery; James L. D. Smith; and Stephen J. O'Brien. 2007. "Development of Y Chromosome Intraspecific Polymorphic Markers in the Felidae." Journal of Heredity 98, (5): 400-413. https://nsuworks.nova.edu/cnso_bio_facarticles/500

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015