Biology Faculty Articles

Title

A Population Genetic Database of Cat Breeds Developed in Coordination with a Domestic Cat STR Multiplex

Document Type

Article

Publication Date

5-2012

Publication Title

Journal of Forensic Sciences

Keywords

Forensic science, Domestic cat, Simple tandem repeat, Forensic typing system Population genetic database, Cat breeds

ISSN

0022-1198

Volume

57

Issue/No.

3

First Page

596

Last Page

601

Abstract

A simple tandem repeat (STR) PCR-based typing system developed for the genetic individualization of domestic cat samples has been used to generate a population genetic database of domestic cat breeds. A panel of 10 tetranucleotide STR loci and a gender-identifying sequence tagged site (STS) were co-amplified in genomic DNA of 1043 individuals representing 38 cat breeds. The STR panel exhibits relatively high heterozygosity in cat breeds, with an average 10-locus heterozygosity of 0.71, which represents an average of 38 breed-specific heterozygosities for the 10-member panel. When the entire set of breed individuals was analyzed as a single population, a heterozygosity of 0.87 was observed. Heterozygosities obtained for the 10 loci range from 0.72 to 0.96. The power for genetic individualization of domestic cat samples of the multiplex is high, with a probability of match (pm) of 6.2E-14, using a conservative θ = 0.05.

Comments

©2012 American Academy of Forensic Sciences

Additional Comments

National Institute of Justice Interagency grant #: IAA #1999-IJ-R-A079); National Institute of Health grant #: GM 75091

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

This document is currently not available here.

Find in your library

Share

COinS