Title

Characterization of WiDr: A Human Colon Carcinoma Cell Line

Authors

P. Noguchi, Bureau of Biologics
R. Wallace, Lederle Laboratories
J. Johnson, Bureau of Biologics
E. M. Earley, Bureau of Biologics
Stephen J. O'Brien, National Cancer Institute at BethesdaFollow
S. Ferrone, Scripps Clinic and Research Foundation
M. A. Pellegrino, Scripps Clinic and Research Foundation
J. Milstien, Bureau of Biologics
C. Needy, Bureau of Biologics
W. Browne, Bureau of Biologics
J. Petricciani, Bureau of Biologics

Document Type

Article

Publication Date

6-1979

Publication Title

In Vitro

Keywords

Human tumor cell line, HLA antigens, Allozyme genetic signature, Karyology, Tumorigenicity

ISSN

0073-5655

Volume

15

Issue/No.

6

First Page

401

Last Page

408

Abstract

We describe the establishment and characterization of WiDr, a cell line derived from a human colon carcinoma. It produces carcinoembryonic antigen in culture, and has a doubling time of 15 hr with plating efficiency of 51%. The HLA antigenic profile and the allozyme genetic signature (composed of eight gene-enzyme systems) of WiDr cells are different from those of HeLa cells. Furthermore, WiDr cells possess three marker chromosomes, again distinct from the HeLa marker chromosomes. Finally, it is highly tumorigenic in four different xenogeneic animal models. Based on these studies, WiDr represents a useful model cell line for tumor cell biology investigations.

Comments

©1979 In Vitro. All rights reserved.

NSUWorks Citation

Noguchi, P.; R. Wallace; J. Johnson; E. M. Earley; Stephen J. O'Brien; S. Ferrone; M. A. Pellegrino; J. Milstien; C. Needy; W. Browne; and J. Petricciani. 1979. "Characterization of WiDr: A Human Colon Carcinoma Cell Line." In Vitro 15, (6): 401-408. doi:10.1007/BF02618407.

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

DOI

10.1007/BF02618407