Biology Faculty Articles

Title

Cloning and Comparative Mapping of a Human Class III (X) Alcohol Dehydrogenase cDNA

Document Type

Article

Publication Date

10-16-1989

Publication Title

Biochemical and Biophysical Research Communications

ISSN

0006-291X

Volume

164

Issue/No.

1

First Page

453

Last Page

460

Abstract

A cDNA encoding human class III (χ, ADH5) alcohol dehydrogenase was isolated, sequenced and used to comparatively map this unusual ADH. In their coding sequences, the three major ADH classes were approximately equisimilar, class II and III ADHs sharing the highest sequence identity (67%). A class III-like ADH was mapped to mouse chromosome 3, site of the ADH gene complex, and synteny of ADH5 with four other ADH loci on human chromosome 4 was confirmed. The nearly full-length 1613 nucleotide cDNA contained 433 nucleotides of 3′ nontranslated sequence and two possible initiation sites for translation. A protein of 374 amino acid residues could be synthesized using the potential initiation codon at nucleotide 59. However, use of the likely initiation codon at nucleotide 5 would produce a protein of 392 residues with 19 additional N-terminal residues as compared to the known protein sequence. The derived protein sequence also differs at residue 166, where Tyr is found. This difference, due to a single base substitution, could result from cloning artifact, polymorphism, or two expressed class III ADH genes.

Comments

© 1989 Published by Elsevier Inc.

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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