Biology Faculty Articles

Title

A Glutaminase (Gls) Gene Maps to Mouse Chromosome 1, Rat Chromosome 9, and Human Chromosome 2

Document Type

Article

Publication Date

8-1989

Publication Title

Genomics

ISSN

0888-7543

Volume

5

Issue/No.

2

First Page

291

Last Page

297

Abstract

A rat cDNA clone encoding a portion of phosphate-activated glutaminase was used to identify DNA restriction fragment length polymorphisms (RFLPs) in sets of somatic cell hybrids and between wild-derived and inbred strains of mice. Segregation of rat and mouse chromosomes among somatic cell hybrids indicated assignment to rat chromosome 9 and mouse chromosome 1. Analysis of chromosome 1 alleles for several genes in an interspecific cross between Mus spretus and C3H/HeJ - gld/gld mice indicates that glutaminase can be positioned within 5.5 ± 2.0 cM proximal to Ctla-4. Similarly, human-hamster somatic cell hybrids were examined for RFLPs, and four human EcoRI restriction fragments were found to hybridize with the rat glutaminase probe. Two of these restriction fragments cosegregated and mapped to human chromosome 2 in a region that is syntenic with mouse chromosome 1 and rat chromosome 9.

Comments

© 1989 Published by Elsevier Inc.

Additional Comments

GenBank accession #: M22586

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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