Biology Faculty Articles

Title

Influence of Temperature and Gas Atmosphere on In-Vitro Fertilization and Embryo Development in Domestic Cats

Document Type

Article

Publication Date

7-1-1991

Publication Title

Journal of Reproduction and Fertility

Keywords

Cat, Fertilization, Embryo, In vitro culture, Incubation, Temperature, Gas atmosphere

ISSN

0022-4251

Volume

92

Issue/No.

2

First Page

377

Last Page

382

Abstract

The influence of culture temperature and gas atmosphere on in-vitro fertilization and embryo development was examined in the domestic cat. In Exp. 1, eggs were fertilized and cultured in 5% CO2 in air at 37, 38 or 39°C. Experiment 2 evaluated the effects of 5% CO2 in air; 5% CO2, 5% O2 and 90% N2; and 10% CO2 in air. Fertilization (cleavage) and development to the morula/blastocyst stage were not influenced (P > 0·05) by variations in temperature and gas composition. Despite changing these culture conditions, egg cleavage averaged ∼75% and >80% of the 2-cell embryos proceeded to morulae in vitro. However, the partial in-vitro morula-to-blastocyst developmental block normally observed in this species was not removed.

Comments

©1991 Journals of Reproduction and Fertility Ltd

Additional Comments

Nationa Institutes of Health grant #: NIH HD 23583

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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