Biology Faculty Articles

Title

Mapping of PRM 1 to Human Chromosome 16 and Tight Linkage of Prm-1 and Prm-2 on Mouse Chromosome 16

Document Type

Article

Publication Date

9-1989

Publication Title

Journal of Heredity

ISSN

0022-1503

Volume

80

Issue/No.

6

First Page

442

Last Page

446

Abstract

The protamines are small, arginine-rich nuclear proteins that replace histones and transition proteins late in the haploid phase of spermatogenesis in mammals. The two mouse genes encoding protamines– Prm-1 and Prm-2– have been molecularly cloned and mapped to mouse chromosome 16 (MMU 16). A cDNA clone of mouse Prm-1 that hybridized to the corresponding human gene was used to analyze a panel of somatic cell hybrids made between human lymphoblasts and the E36 hamster cell line. The human gene, which we have designated PRM1, was syntenic with human chromosome 16 (HSA 16) and discordant with all other human chromosomes. Linkage analysis in the mouse was accomplished using the backcross (Czech II × BALB/c Pt) × Czech II to map Prm-1 and Prm-2 to a position near the 5’ terminus of MMU 16. No recombination between Prm-1 and Prm-2 was observed among 89 progeny of the Czech II × BALB/c cross or among 94 progeny of the backcross (CBA/J × BALB/cJ) × BALB/cJ, demonstrating that the two loci are separated by less than 1.6 cM on MMU 16. This tight linkage may be of functional significance, as Prm-1 and Prm-2 are among a limited number of genes known to be expressed postmeiotically in male haploid germ cells.

Comments

©1990 The American Genetic Association

Additional Comments

Public Health Service Grant #s: PO1 HD19920, RO1 HD22262, GM29224

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

This document is currently not available here.

Find in your library

Share

COinS