Influence of Culture Medium and Protein Supplementation on In Vitro Oocyte Maturation and Fertilization in the Domestic Cat
Cat, Oocyte maturation, Fertilization, Embryo
Domestic cat oocytes were cultured either in Waymouth MB 753/1 Medium (WAY) or in Eagle's Minimum Essential Medium (MEM) containing FSH, LH and estradiol-17β and supplemented with one of the following: 5% fetal calf serum (FCS); 4 mg/ml bovine serum albumin (BSA); or 3 mg/ml polyvinylalcohol (PVA, a non-protein control). The oocytes were evaluated for: nuclear maturation after 48 hours of culture (in vitro maturation, IVM); fertilization and cleavage 24 to 30 hours postinsemination (in vitro fertilization, IVF); and early embryo development 48 hours postinsemination. Maturation rates were similar (P>0.05) for WAY + BSA (29.4%), MEM + BSA (46.7%) and MEM + PVA (43.3%), but were different (P0.05) for WAY + BSA (51.4%, 30.5%), MEM + BSA (45.8%, 40.1%) and MEM + PVA (56.1%, 37.4%) and were greater (Pin vitro can proceed in this species in the absence of supplementary protein.
Johnston, L. A.; A. M. Donoghue; Stephen J. O'Brien; and David E. Wildt. 1993. "Influence of Culture Medium and Protein Supplementation on In Vitro Oocyte Maturation and Fertilization in the Domestic Cat." Theriogenology 40, (4): 829-839. http://nsuworks.nova.edu/cnso_bio_facarticles/276