Biology Faculty Articles

Title

Molecular Cloning and Chromosomal Mapping of Feline p53 Tumor Suppressor Gene

Document Type

Article

Publication Date

1993

Publication Title

Journal of Veterinary Medical Science

ISSN

0916-7250

Volume

55

Issue/No.

6

First Page

801

Last Page

805

Abstract

Alterations of the p53 tumor suppressor gene have been observed in a variety of human and mouse tumors. For investigation of the role of this gene in tumors in cats, feline p53 cDNA was molecularly cloned by PCR amplifications using primers based on the sequences conserved among several species. The cloned cDNA appeared to cover approximately 90% of the open reading frame of the feline p53 gene and had characteristic structures in common with the p53 genes of several other species. The amino acid sequence similarities of the feline p53 with the human, mouse, rat and chicken counterparts were 82.9%, 75.6%, 76.5% and 57.2% respectively. Moreover, using a panel of feline x rodent somatic cell hybrids, the feline p53 gene was assigned to feline chromosome E1. These data will be useful for determining the role of the p53 tumor suppressor gene in feline tumors.

Comments

©Japanese Society of Veterinary Science

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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