Biology Faculty Articles

Title

Assignment of Islet Amyloid Polypeptide (IAPP) Gene to Feline Chromosome B4 Using the Polymerase Chain Reaction Technique on Feline-Rodent Hybrid Cell Lines

Document Type

Article

Publication Date

3-1995

Publication Title

Veterinary Pathology

Keywords

Chromosome, Diabetes mellitus, Islet amyloid polypeptide, Polymerase chain reaction

ISSN

0300-9858

Volume

32

Issue/No.

2

First Page

195

Last Page

197

Abstract

The most characteristic morphologic features of the pancreatic islets of human non-insulin-dependent diabetes mellitus (NIDDM, or type 2 diabetes mellitus) and of similar forms of diabetes in cats and macaques are the deposition of amyloid (islet amyloid) and the loss of beta cells. Islet amyloid is derived from islet amyloid polypeptide (IAPP), which is a normal secretory product of the beta cells. Therefore, knowledge of the IAPP gene is of potential importance in defining the pathogenesis of NIDDM. To identify the feline chromosome(s) on which the IAPP gene is located, we screened genomic DNA obtained from 38 feline-rodent hybrid cell lines that have a known feline chromosome content. Feline IAPP DNA was amplified and detected using the polymerase chain reaction technique. Discordancy analysis for each feline chromosome showed that chromosome B4 had the lowest discordancy (P < 0.0001). Feline chromosome B4 shows an extensive conserved syntenic relationship with human chromosome 12, on which the human IAPP gene is located. This study therefore extends and confirms the homology between human chromosome 12 and feline chromosome B4 and provides an additional genetic marker for feline chromosome B4.

Comments

©1995 by American College of Veterinary Pathologists

Additional Comments

National Institute of Diabetes and Digestive and Kidney Disease grant #:R01 DK36734

ORCID ID

0000-0001-7353-8301

ResearcherID

N-1726-2015

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