Isolation of HTLV-Transformed B-Lymphocyte Clone from a Patient with HTLV-Associated Adult T-Cell Leukaemia
The human T-cell leukaemia/lymphoma virus (HTLV) is an exogenous retrovirus which has been associated with adult T-cell leukaemia/lymphoma (ATL). This malignancy of T lymphocytes is endemic to southern Japan, the West Indies, and to a lesser extent, the Middle East, Central Africa and the southeastern United States. ATL cells from patients of diverse geographical origins have been found to be infected with HTLV-1. HTLV is normally tropic for mature T lymphocytes, especially those expressing the helper-inducer surface antigen phenotype (OKT4 or Leu-3-positive), and the neoplastic T cells infected with HTLV generally express receptors for T-cell growth factor (detected by reactivity with anti-Tac antibody). However, we report here the isolation of a HTLV-infected B-lymphocyte clone from the peripheral blood of a patient with ATL. This clone is cytogenetically normal and is not infected with Epstein-Barr virus (EBV). Co-culture of cells from this clone with cord blood lymphocytes resulted in transmission of HTLV and the immortalization of either T or B lymphocytes. These results suggest that HTLV may be associated with a broader range of host cells than previously recognized.
Longo, Dan L.; Edward P. Gelmann; Jeffrey Cossman; Rebecca A. Young; Robert C. Gallo; Stephen J. O'Brien; and Louis A. Matis. 1984. "Isolation of HTLV-Transformed B-Lymphocyte Clone from a Patient with HTLV-Associated Adult T-Cell Leukaemia." Nature 310, (5977): 505-506. http://nsuworks.nova.edu/cnso_bio_facarticles/163